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What is vacuum blood collection tube?

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What is vacuum blood collection tube?

A vacuum blood collection tube is a sterile glass or plastic test tube with a colored rubber stopper to create a vacuum-sealed interior of the tube, promoting a diagram of a predetermined volume of liquid. The vacuum tube may contain additives designed to stabilize and preserve the sample before analytical testing. The test tube has a safety-designed stopper, and comes with a variety of label options and withdrawal volumes. The color on the top indicates the additive in the vial.

This passage is going to talk about the following questions of vacuum blood collection tube:

(1) What is vacuum blood collection tube?

(2) Principle of vacuum blood collection tube

(3) Types of vacuum blood collection tube

(1) What is vacuum blood collection tube?

The vacuum blood collection tube is a one-time negative pressure vacuum glass tube that can realize quantitative blood collection and needs to be used in conjunction with a venous blood collection needle. It has the characteristics of safety, long specimen maintenance time and good accuracy.

(2) Principle of vacuum blood collection tube

Use a vacuum pump to draw blood. The clear plastic sheath around the top of the tube secures the needle. After the needle is pulled out from the patient, the orange protective cover will protect the needle to prevent accidental needle sticks.

The Vacutainer needle is double-ended: the inner end is wrapped with a thin rubber coating. If the Vacutainer needle tube is replaced during multiple suctions, it can prevent blood leakage, while the outer end is inserted into a vein. When screwing the needle into the translucent plastic needle holder, the coated end is inside the holder. When a tube is inserted into the holder, its rubber cap is pierced by the inner needle, and the vacuum in the tube draws blood through the needle and into the tube. Then remove the filled tube, and then insert and fill another tube in the same way. The amount of air expelled from the test tube determines how much blood will fill the test tube before the blood stops flowing.

A color-coded plastic or rubber cap is attached to the top of each test tube. Test tubes usually contain additives that mix with blood when added, and the color of the plastic cap of each test tube indicates which additives are contained in the test tube.

As time goes by, the vacuum will disappear, and blood will not be sucked into the blood collection tube when the needle pierces the cap, so the blood collection tube gradually fails.

(3) Types of vacuum blood collection tube

The centrifuged tiger top tube separates hemorrhagic cells from the serum

Vacuum tubes may contain other substances that can save blood for processing in medical laboratories. Using the wrong test tube may prevent the blood sample from being used for the intended purpose. These additives are usually thin film coatings applied using ultrasonic nozzles.

Additives may include anticoagulants (EDTA, sodium citrate, heparin) or gels with a density between blood cells and plasma. In addition, some test tubes contain additives that retain certain components or substances in the blood (such as glucose). When the tube is centrifuged, the contents are separated by density, blood cells sink to the bottom, and plasma or serum accumulates on the top. The test tube containing the gel can be easily handled and transported without mixing blood cells and serum after centrifugation.

The term "pumping sequence" refers to the sequence in which the tubes should be filled. The needle that pierces the tube can carry additives from one tube to another, so the sequence is standardized, so any cross-contamination of additives will not affect laboratory results.

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