Views: 0 Author: Site Editor Publish Time: 2021-06-01 Origin: Site
Cryopreservation, the preservation of biomaterials at a sufficiently low temperature to prevent cell division while maintaining integrity and stability, is the basis of modern biological experiments. In addition to the selection of equipment and methods, the selection of appropriate storage tubes is equally important for success. Proper use and low-temperature preservation ensure that the material remains unchanged from the first harvest, and reduce the variability of the sample before the experiment by ensuring the quality of the sample.
Conventional cryopreserved samples include biological fluids, such as whole blood or plasma, cell cultures, tissue samples, and genomic materials, such as DNA. In addition to a clear understanding of cryopreservation technology, it is also necessary to understand the biological samples and their intended uses.
How do you choose? Here are some factors that can help you.
Glass or Plastic?
It is worth mentioning that the cooling rate of glassware is faster than that of plastic, which may also be related to the thawing of biological samples.
It is important to ensure that the sample is cooled at an appropriate rate when cells are cryopreserved. The cooling rate is about - 1°C provides optimal dehydration per minute and reduces water content in cells, which can form destructive ice crystals. Although most cells and tissues are well adapted to freezing, embryos, as multicellular specimens, need special care during cryopreservation and thawing; Therefore, it is very important to choose the right storage tube. Superfit's Freezing Cryogenic Vial is designed for the best user experience and convenience.
Another factor to consider is the storage time: how long do you want to keep the sample? To avoid sample loss, check whether the pipe you are using is durable and can withstand low-temperature storage without damage.
Vial, Ampoule, Straw, or Plate?
The most commonly used ampoules and vials are about 1.2 ml to 2 ml in volume and are suitable for samples between 0.5 ml and 1 ml. One factor to consider is whether a test tube with a screw cap (internal or external thread) or bottle cap is required to close the container.
It's also important to consider the type of samples you're going to work with: how do you store and retrieve them? For example, embryos are usually stored in pipettes, while some materials are more suitable to be placed in microtitration plates, and biological fluids are usually equally placed in sample tubes.
Other considerations include technology and treatment; For example, glass ampoules may require appropriate heat treatment to seal the contents individually, while multi-tube systems benefit from automatic batch capping systems.
Low or Ultra Low: Liquid Nitrogen or Refrigerator?
The biological samples preserved in liquid nitrogen and cryopreservation have different needs. Ideally, keep the sample in the gaseous phase of liquid nitrogen to avoid contamination and minimize temperature fluctuations. Choose low-temperature-resistant ampoules and vials, which can withstand extremely low temperatures without cracking.
If the glass is used, make sure that the seal burns correctly. This avoids leaving a microchannel that allows liquid nitrogen to enter, which is not only a pollution source but also a risk of explosion due to the rapid expansion of gas during thawing.
In short, choosing the right storage medium for your samples is an important first step in cryopreservation. The investment of time and energy in this step can not only maximize the survival ability and recovery rate but also enable researchers to benefit from the reliable acquisition of high-quality samples.