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Views: 0 Author: Site Editor Publish Time: 2021-09-16 Origin: Site
I'm sure you've seen the term "embedding" or heard it mentioned by friends in the laboratory. Those who are unfamiliar with it, on the other hand, may be perplexed by embedding. To help you understand embedding, we will briefly discuss the embedding technique, why we need embedding, and the common media and needs of embedding in this post. If you learned something from this post, please share it with your friends and let them be amazed by what you have learned.
Embedding is the process of enclosing tissues or specimens in a mass of embedding media using a mould. Because the tissue blocks are so thin, they require a supporting medium in which they can be embedded. This supporting media is known as the embedding medium. Paraffin wax, celloidin, synthetic resins, gelatine, and other embedding ingredients are examples.
1.Embedding is simple and can be accomplished by merging the cells with monomers or polymers and embedding the cells in the produced polymer.
2.The embedding conditions are gentle, and alternative polymer carriers, embedding systems, and settings can be used to preserve the cells' enzymatic catalytic activity.
3.Cells from embedding samples are less permeable, more stable, and have a high cell volume, with up to 50-70 percent cell content in the polymer.
The type of embedding media used is determined by:
Type of microscope
Type of microtome
Type of tissue, for example, hard tissue like bone or soft tissue like liver biopsy
For embedding, paraffin waxes with a higher melting point (56 to 62°C) are utilized. In the oven, the molten wax is filtered through a layer of filter paper into another container. This guards the knife's edge.
Celloidin is a purified form of nitrocellulose. It is used to cut through tough tissue.
Electron microscopy z makes use of epoxy resin. In double embedding and FNAC specimens, agar embedding is employed. Celloidin medium is used to sever hard tissues. When frozen pieces of friable tissues are required, gelatin is used.
Gelatin is insoluble in cold water, but once wet, it slowly absorbs water, swells, and softens, and can absorb 5 to 10 times its weight in water. In hot water, gelatin dissolves and creates a thermally reversible gel with water. Because gelatin is both a hydrophilic colloid and an amphiphilic electrolyte, it can be employed as a stabilizer and emulsifier for hydrophobic colloids, as well as a protective colloid; gelatin is also fully non-toxic, and its toxicity is not addressed when used as an embedding carrier.
Superfit's disposable plastic Embedding Cassettes are made of acetal. These cassettes keep specimens safely submerged in liquid and are histologically resistant. Please Contact Us if you are interested.
