Views: 0 Author: Site Editor Publish Time: 2021-06-08 Origin: Site
The best way to get the best results through a microscope is to make sure that your research sample is fixed in such a way that the light passes through it evenly and the important features of the sample are divided. So you should：
Choose the highest quality glass for your slides and coverslips.
Choose slides that are suitable for your special microscope equipment and examination purpose.
Use a suitable slide mount.
Use an effective mounting medium.
Choose the right stain to show the desired function.
The first element to get the highest quality slides is to choose the best microscope slide. Some of the glasses in the microscope slide contain impurities. These pollutants floating in the glass may produce false color or fluorescence, which will reduce the contrast and clarity.
The microscope slides must be made of optical quality glass. Some of the best microscope slides are made of soda-lime glass or borosilicate glass. Sometimes transparent plastics of special optical quality are used.
The cover glass used for flat specimen or protecting microscope lens shall be made of the best quality optical glass, as thin as possible to reduce the film effect.
Glass slides are usually of special shapes for different purposes. The concave slide has a shallow well on the upper surface to hold the liquid. Raster slides have measurement grids etched onto the surface to help cell count or assess the size of the object. Some slides have special reservoirs and channels cut.
Use fused silica instead of glass when fluorescent lamps will be used to illuminate the body.
The word "mount" sometimes sounds a bit exaggerated. Mounting is simply putting the sample on the slide. For at least 200 years, the art and science of slide mounting have developed to improve microscopy. The way samples are installed on a slide often distinguishes clear images from confusing and confusing images.
Dry mounting is the simplest mounting method. It just puts the thin, light transmission subject on the slide. Usually, a cover slide is placed on the top to keep it flat and keep the objective of the microscope from contaminating the subject.
A wet or temporary mount means that the subject is immersed in water or another liquid on a slide. Wet mounts are usually used for liquids related to the nature of the subject (e.g., watching swimming protozoa). Cover slide is almost always used to flatten the object and avoid the refraction effect of the liquid.
The mounting medium can be water. Sometimes glycerol (slightly denser than water) is used.
The permanent mounting usually adopts specially designed materials to keep the thickness and fixation of the specimen for a long time, to reduce corrosion and pollution.
There are many popular types of installation media.
The mounting medium shall not act as a lens as this will increase additional light refraction and distortion. The installation medium must be selected so that its refractive index is the same as that of the sample. If the two refractive indices are too different, the points where the medium strikes the sample will be shown as dark areas, which can hide important details.
Stains will be combined with special features of cell anatomy to make them more visible under the microscope. There are many kinds of stains with different uses. The quality of your slides usually depends on the proper use of stains.
Superfit provides the highest quality microscope glass slides in the industry. Please contact us for more information.